The collection consists primarily of Gambell's written reminiscences. These include published newspaper accounts of his time in Alaska, as well as short stories and poetry based on his experiences in Alaska and as a doctor in California. Gambell also wrote about his childhood in Iowa, his years at Parsons College, and his decision to choose a career in medicine.

In addition, the collection includes news clippings about Gambell, and the transcript of an interview with him about his experiences. It contains a diary (1899) which Gambell kept in Alaska, a scrapbook from his time in Siberia, and drafts of introductions to a book or article that Gambell apparently wrote about Alaska.